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辣椒RUB激活酶和结合酶基因家族的全基因组鉴定及其在非生物胁迫下的表达分析
Protoplasma
(
IF
2.5
)
Pub Date : 2022-11-02
, DOI:
10.1007/s00709-022-01816-4
Min Wei
1
,
Panpan Duan
1
,
Shufang Zhao
1
,
Bingdiao Gou
1
,
Yongfu Wang
1
,
Nan Yang
1
,
Yan Ma
1
,
Zhengbao Ma
1
,
Gaoyuan Zhang
1
,
Bingqiang Wei
1
Affiliation
- College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, People's Republic of China.
NEDD8/RUB 作为一种泛素样蛋白,参与蛋白质的翻译后修饰,需要独特的 E1、E2 和 E3 酶与其底物结合。RUB E1 激活酶和 E2 结合酶在 neddylation 中起重要作用。但是,胡椒中是否存在RUB E1和E2 ,其作用是什么,目前还不得而知。在这项研究中,在辣椒基因组中总共鉴定了三个推定的RUB E1和五个RUB E2基因。随后,分析了它们的理化性质、基因结构、保守结构域和基序、系统发育关系和顺式作用元件。RUB E1和的结构和保守域E2与拟南芥和番茄相似。RUB E1和E2基因随机分布在7条染色体上,辣椒与拟南芥存在2对共线性,辣椒与番茄存在8对共线性。系统发育分析表明,辣椒的RUB E1、E2基因与番茄、马铃薯、烟草的关系更为密切。RUB E1和E2基因的顺式元件含有激素反应和应激反应。擦 E1和E2基因在至少一种组织中表达,CaRCE1.3和CaRCE2.1仅在花和花药中表达。此外,在低温、干旱、_ _ _ _ _ _ _ _ _ _盐、ABA 和 IAA 处理,而CaRCE1.3和CaRCE2.2在低温处理下被下调。此外,这些基因在 MeJA 处理下几乎不表达。综上所述,本研究为探索RUB E1和E2在植物胁迫响应中的作用提供了理论基础。
"点击查看英文标题和摘要"
Genome-wide identification of RUB activating enzyme and conjugating enzyme gene families and their expression analysis under abiotic stresses in Capsicum annuum
NEDD8/RUB, as a ubiquitin-like protein, participates in the post-translational modification of protein and requires unique E1, E2, and E3 enzymes to bind to its substrate. The RUB E1 activating enzyme and E2 conjugating enzyme play a significant role in the neddylation. However, it is unknown whether RUB E1 and E2 exist in pepper and what its function is. In this study, a total of three putative RUB E1 and five RUB E2 genes have been identified in the pepper genome. Subsequently, their physical and chemical properties, gene structure, conserved domains and motifs, phylogenetic relationship, and cis-acting elements were analyzed. The structure and conserved domain of RUB E1 and E2 are similar to that of Arabidopsis and tomato. The RUB E1 and E2 genes were randomly distributed on seven chromosomes, and there were two pairs of collinearity between pepper and Arabidopsis and eight pairs of collinearity between pepper and tomato. Phylogenetic analysis reveals that RUB E1 and E2 genes of pepper have a closer relationship with that of tomato, potato, and Nicotiana attenuate. The cis-elements of RUB E1 and E2 genes contained hormone response and stress response. RUB E1 and E2 genes were expressed in at least one tissue and CaRCE1.3 and CaRCE2.1 were exclusively expressed in flowers and anthers. Moreover, the expression of RUB E1 genes (CaECR1, CaAXR1.1, and CaAXR1.2) and RUB E2 genes (CaRCE1.1, CaRCE1.2, and CaRCE2.1) was increased to varying degrees under low-temperature, drought, salt, ABA, and IAA treatments, while CaRCE1.3 and CaRCE2.2 were down-regulated under low-temperature treatment. In addition, these genes were hardly expressed under MeJA treatment. In summary, this study provides a theoretical foundation to explore the role of RUB E1 and E2 in the response of plants to stress.
更新日期:2022-11-02